胞内劳森菌TaqMan荧光定量PCR检测方法的建立

doi:10.11843/j.issn.0366-6964.2014.10.021

Abstract

Abstract:

Based on the 16S rDNA，specific primers and probe were designed，and a TaqMan quantitative polymerase chain reaction assay (TaqMan-PCR) was developed to determinate Lawsonia intracellularis.The results showed that the correlation equation of this TaqMan-PCR method was Ct=-3.318×log(conc)+38.840，and had a good linear relativity (R²=0.998).The TaqMan-PCR prove to be a sensitive，specific，reproducible，and accurate method，and can detecting as little as 5.55 copies•μL^－1 standard plasmid with 16S rDNA of L.intracellularis. The new TaqMan-PCR could be applied for qualitative and quantitative detection of L.intracellularis from clinical sample and the evaluation of treatment effect.

CLC Number:

S852.613

BAI Ai-quan，GUO Jian-chao，QIN Zong-hua，PU Wen-jun，MA Chun-quan，LI Guo-qing，ZHANG Hao-ji. Development of a TaqMan Quantitative Polymerase Chain Reaction Assay for Detecting Lawsonia intracellularis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1733-1738.

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References

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Development of a TaqMan Quantitative Polymerase Chain Reaction Assay for Detecting Lawsonia intracellularis

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